Alveolar Macrophages Infected with Ames or Sterne Strain of Bacillus anthracis Elicit Differential Molecular Expression Patterns

Alveolar Macrophages Infected with Ames or Sterne Strain of Bacillus anthracis Elicit Differential Molecular Expression Patterns

Alveolar macrophages (AMs) phagocytose Bacillus anthracis following inhalation and induce the production of pro-inflammatory cytokines and chemokines to mediate the activation of innate immunity. Ames, the virulent strain of B. anthracis, contains two plasmids that encode the antiphagocytic poly-γ-d-glutamic acid capsule and the lethal toxin. The attenuated Sterne strain of B. anthracis, which ...

Stability and potency studies of anthrax vaccine (Bacillus anthracis 34F2 Sterne strain) in Iran

  For evaluation the stability and potency of anthrax vaccine prepared in Razi Vaccine and Serum Research Institute in Iran, samples of different batches of vaccine were kept at 4-8 °C (refrigerator), 20-25 °C (room temperature) and 37 °C (incubator).The viable spores/ml of vaccines were determined using plate–counting method before and after holding at different temperatures monthly. Vaccine p...

Behavior of Bacillus anthracis strains Sterne and Ames K0610 in sterile raw ground beef.

The behavior of Bacillus anthracis Sterne spores in sterile raw ground beef was measured at storage temperatures of 2 to 70 degrees C, encompassing both bacterial growth and death. B. anthracis Sterne was weakly inactivated (-0.003 to -0.014 log10 CFU/h) at storage temperatures of 2 to 16 degrees C and at temperatures greater than and equal to 45 degrees C. Growth was observed from 17 to 44 deg...

A Study on molecular characterization of Razi Bacillus anthracis Sterne 34F2 substrain in Iran

Anthrax, a zoonotic disease caused by Bacillus anthracis, has affected humans since ancient times. For genomic characterization of Razi B. anthracis Sterne 34F2 substrain, single nucleotide polymorphism (SNP) genotyping method developed by Van Erth, variable-number tandem-repeat (VNTR)-8 analysis proposed by Keim, and multiple-locus VNTR analysis (MLVA)-3 introduced by Levy were employed. In th...

Lethal Toxin Bacillus anthracis to Resistance of Human Alveolar Macrophages